MOLECULAR EVALUATION OF THE ANTI DIABETIC EFFECT OF AQUEOUS EXTRACT OF SACCHARUM OFFICINARUM STEM BARK(SUGAR CANE) IN WISTAR RAT
Aim: To evaluate the anti-diabetic effect of aqueous extract of Saccharum officinarum (stem bark) in the genomic DNA of diabetic Wistar rats.
Methodology: 15 Streptozotocin-induced diabetic male rats and five non diabetic rats were randomly and evenly distributed into four groups, and were subsequently exposed to the following treatments for 14 days: Group I (Control): Distilled water (1ml/kg B.W); Group II: Normal saline (1ml/kg B.W); Group III: treated Diabetic rats (Metformin) (500mg/kg B..W); Group IV: Diabetic rats treated with Saccharum officinarum (stem bark) (500mg/Kg B.W). Administration was done orally for 14 days. Body weight changes were analyzed by induction of obesity through high fat diet. Diabetes type 2 was induced using Streptozotocin injected intraperitoneally (40mg/kg B.W). The serum fasting blood glucose was analyzed using the glucose test kits (AGGAPPE). The liver tissue from each group was randomly selected and analyzed using the Nano drop spectrophotometric analysis.
Result: The body weight of the rats were significant after twelve weeks of high fat diet (p< 0.05). Fasting blood glucose level changes in diabetic treated rats (p<0.002), there were changes in genomic DNA yield and purity in the diabetic treated rats (p<0.05)
Conclusion: The aqueous extract of the plant showed anti-diabetic potentials by decrease in the fasting blood glucose level and causing significant changes in genomic DNA quality and quantity