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QUANTITATIVE DETERMINATION OF CARBAZOLE ALKALOIDS FROM CRUDE PLANT EXTRACT (Glycosmis pentaphylla of family Rutaceae) USING GAS LIQUID CHROMATOGRAPHY
Abstract:
Quantitation of carbazole alkaloids (Carbazole, 3-Methyl Carbazole.Glycozoline and Glycozolidine) was carried out using Gas-Liquid Chromatography (GLC ). This was -the first time a quantitative work was undertaken on carbazole alkaloids© A stock solution of 1mg/ml in chloroform was prepared of each of the above mentioned carbazole alkaloids© Appropriate working concentration were prepared by suitable dilutions of various stock solutions .with chloroform. Heptazolidine , (lmg/ml) in chloroform, was used as the internal standard (i.s.) The plant extract (Glycosmis Pentaphylla of family Rutaceas) solution (unknown sample) was prepared by drawing a drop (weighed 23mg) from the light Petroleum extract by using a clean glass rod and diluting it to 5ml with chloroform. The I.S. (1ml) was added to all solutions prior to analysis© All samples (3ul) were chromatographed on a Bye Unicam series 204 chromatograph equipped with FID and Temperature programmer© A single point internal standard method was employed for the analysis of result. TLG was performed of carbazole, 3 – methyl carbazole and the Crude plant extract© Single point method employed was faster and more convenient that the traditional individual calibration curve method. The concentrations of the alkaloids were found as:- 1. Carbazole – 17.35 ug/ml (0.4% w/w) 2. 3-Methyl Carbazole – 186.67 • (4.06% * ) 4. Glycozolidine – 189.70 ” (4.1 2% ” ) plant TLC results added supportive evidence that the/extract contains carbazole and 3 – methyl-carbazole (all identified for the first time from this plant species)c G-LC was found to be a very convenient means of finding the levels of components in a plant extract.
QUANTITATIVE DETERMINATION OF CARBAZOLE ALKALOIDS FROM CRUDE PLANT EXTRACT (Glycosmis pentaphylla of family Rutaceae) USING GAS LIQUID CHROMATOGRAPHY
