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CONTAMINATION OF CHICKEN CARCASSES AND POULTRY PROCESSING ENVIRONMENTWITH LISTERIA SPECIES IN ZARIA, NIGERIA
Abstract:
Listeria species are widely distributed in the environment and L. monocytogenes is the causativeagent of listeriosis. This study was carried out to isolate and identifyListeria species from chicken carcasses and poultry processing environment, determine the antimicrobial susceptibilitypattern of Listeriaisolates to 12 commonly used antimicrobial agents and to determine haemolysin A (hyl)virulence gene carriage in L. monocytogenesisolatesusing polymerase chain reaction.A total of 412 samples were collected which comprises 308 from chicken carcasses (154 from broilers and 154 from local chickens) and 104 samples from poultry processing environment. From poultry processing environment, 26 swab samples were collected from surfaces of tables, floor, knives and bowls.Listeria species were isolated using pre-enrichment selective medium and Listeriaspecies(spp) were identified by conventional biochemical tests and confirmed with Microgen ID-System. From the 412 samples, 40 (9.71%) were found to be contaminated with Listeria spp. Out of these 40 isolates, 2 (5%) were found to be L. monocytogenes while the remaining 38 isolates comprisedL. ivanovii 32 (80%), L.grayi4(10%), and L. welshimeri2 (5%) respectively. Distribution of the occurrence of L. monocytogenes by sample type and location showed that the 2 Listeria monocytogenesisolates were from broiler carcasses and one each fromSabo and Danmagaji markets respectively. Similarly, distribution of Listeria species in poultry processing environment showed that 15(14.42%) Listeria species were isolated from poultry processing environment which are distributed as follow knives 1(3.85%), table 1(3.85%), Floor 6(23.08%) and 7(26.92%). Antibiotic susceptibility testing showed that isolates were 100% resistant to amoxicillin, tetracycline, vancomycin and erythromycin while the
CONTAMINATION OF CHICKEN CARCASSES AND POULTRY PROCESSING ENVIRONMENT WITH LISTERIA SPECIES IN ZARIA, NIGERIA
