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PREVALENCE AND ANTIBIOGRAMS OF LISTERIA MONOCYTOGENES ISOLATES FROM RAW MILK AND YOGHURT IN ZARIA, KADUNA STATE, NIGERIA
Abstract:
Listeria monocytogenes is a food borne pathogen frequently associated with the consumption of contaminated food. Severe and life threatening human diseases caused by L. monocytogenes has been reported throughout the world. The present study evaluated the prevalence and antibiograms of Listeria monocytogenes isolated from yoghurt and raw milk sample in Zaria, Kaduna State. One hundred and fifty eight samples each of yoghurt and raw milk making a total of three hundred and sixteen samples were purchased within Zaria, Kaduna State. The samples were bacteriologically analyzed in the laboratory for the presence of L. monocytogenes using conventional biochemical tests and the use of MicrogenTM Listeria -ID System to further confirm the isolates. The conventional biochemical tests identified 36 isolates of Listeria species out of which ten were Listeria monocytogenes from the raw milk samples, giving a prevalence of 3.2%. However, the MicrogenTM Listeria –ID System failed to identify 9 out of the 10 Listeria monocytogenes isolates. Antibiotic susceptibility profiles showed that all the isolates were resistant to three or more antibiotics except for the aminoglycoside (gentamicin) to which all the isolates were susceptible (100%). Nine different resistance patterns were observed with resistance to ampicillin being the most predominant (100%). The minimum Multiple Antibiotic Resistant (MAR) index was 0.27 indicating that isolates were from a source of high antibiotics abuse. The ages of the animals were not significant to the detection of Listeria spp (p=0.346) whereas the pH of the milk was significant to the detection of Listeria spp (p=0.049). The findings of this study suggest the possible role of raw milk in the transmission of antimicrobial resistant strains of Listeria monocytogenes to human.
PREVALENCE AND ANTIBIOGRAMS OF LISTERIA MONOCYTOGENES ISOLATES FROM RAW MILK AND YOGHURT IN ZARIA, KADUNA STATE, NIGERIA
