BACTERIOCIN PRODUCING LACTOBACILLI AND NUGENT SCORE OF WOMEN ATTENDING ANTENATAL CLINIC IN LAGOS UNIVERSITY TEACHING HOSPITAL, NIGERIA

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BACTERIOCIN PRODUCING LACTOBACILLI AND NUGENT SCORE OF WOMEN ATTENDING ANTENATAL CLINIC IN LAGOS UNIVERSITY TEACHING HOSPITAL, NIGERIA

Abstract

Background
Lactobacilli in the vagina protect women from bacterial vaginosis (BV) and other potentially harmful microorganisms. BV is the imbalance in the normal vaginal flora which is predominantly Lactobacilli. It is the most common cause of vaginal discharge and is associated with adverse pregnancy outcome. One of the mechanisms employed by Lactobacilli to fight BV associated organism is the production of an antimicrobial substance known as bacteriocin.
Objectives:
To determine the Nugent score of women attending antenatal clinic in LUTH, isolate and identify Lactobacilli from vagina of the pregnant women, assay for bacteriocins from the Lactobacilli isolated, correlate the production of bacteriocin with Nugent score of the pregnant women, assess the pregnancy outcome of the pregnant women and correlate it with bacteriocin production and Nugent scores
Methods
A total of 300 women who were receiving antenatal care in Lagos University teaching Hospital were recruited in this study. High vaginal swabs were collected from them for assessment of Nugent score to diagnose BV and for culture on MRS agar and blood agar for isolation of Lactobacilli. The Lactobacilli were identified using Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS) and bacteriocin was extracted using the chloroform extraction method.
Result
Assessment of the Nugent scores showed that 32% of the women had Bacterial vaginosis. Lactobacilli isolated from 60.7% of the women and the Lactobacillus isolated was L. acidophilus/gasseri 27.6% followed by L. inners 25.9% others were L. jensenii 22.4% L. debrueckii 15.5% and L. crispatus 8.6%. Bacteriocin production was by 21.1% of the tested. Five Lactobacilli (8.5%) produced both bacteriocin and hydrogen peroxide and the women colonized with these Lactobacilli were all negative for BV and had good pregnancy outcome.
Conclusion
The prevalence of bacterial vaginosis is high among pregnant women in LUTH and the predominant Lactobacillus species is L. acidophilus group followed by L. inners. Lactobacilli and bacteriocin production in combination hydrogen peroxide production correlated with low Nugent score and good pregnancy outcome.

BACTERIOCIN PRODUCING LACTOBACILLI AND NUGENT SCORE OF WOMEN ATTENDING ANTENATAL CLINIC IN LAGOS UNIVERSITY TEACHING HOSPITAL, NIGERIA

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