Biosynthesis of Biologically Active Silver Nanoparticles by Fungal Isolate and Their Antibiotic Potential Against Specific Bacterial Pathogens

Biosynthesis of Biologically Active Silver Nanoparticles by Fungal Isolate and Their Antibiotic Potential Against Specific Bacterial Pathogens

ABSTRACT

The biosynthesis of silver nanoparticles(AgNPs) through biological entities has garnered significant attention due to its eco-friendly and sustainable approach. Fungi, with their diverse metabolites and enzymatic systems, offer a promising avenue for the production of biologically active AgNPs. This study focuses on the biosynthesis of silver nanoparticles by a fungal isolate and evaluates their potential as antibiotics against specific bacterial pathogens. A fungal isolate, identified as [Insert Fungal Species Name] , was cultivated under controlled conditions. The extracellular synthesis of silver nanoparticles was initiated by exposing the fungal culture to a silver precursor solution. The formation and characterization of AgNPs were monitored using UV-Visible spectroscopy, which confirmed the characteristic surface plasmon resonance peak. Further characterization involved Transmission Electron Microscopy (TEM) for size and morphology assessment, Fourier Transform Infrared (FTIR) spectroscopy for functional group analysis, and X-ray Diffraction (XRD) for crystal structure determination. The bioactivity of the synthesized AgNPs was evaluated against a panel of selected bacterial pathogens through well-established antimicrobial assays. The biosynthesis of silver nanoparticles by the fungal isolate was successful, as indicated by the prominent absorbance peak in the UV-Visible spectrum. TEM imaging revealed uniformly distributed nanoparticles with an average size of [Insert Average Size] nm. FTIR spectroscopy demonstrated the involvement of fungal metabolites in nanoparticle synthesis. XRD patterns confirmed the crystalline nature of the AgNPs. In antimicrobial assays, the synthesized AgNPs exhibited significant inhibitory effects against the selected bacterial pathogens, with minimum inhibitory concentrations (MICs) ranging from [Insert MIC Range] µg/mL. The biosynthesis of biologically active silver nanoparticles by the fungal isolate showcases the potential of fungi as sustainable and eco-friendly nano factories. The involvement of fungal metabolites in nanoparticle synthesis suggests a complex biochemical process. The observed antimicrobial activity underscores the potential of these AgNPs as effective antibiotics against specific bacterial pathogens. The size and morphology of the nanoparticles play a crucial role in their bioactivity, influencing interactions with bacterial cells. This study demonstrates the successful biosynthesis of biologically active silver nanoparticles by a fungal isolate and highlights their potential as effective antibiotics against specific bacterial pathogens. The eco-friendly synthesis process, coupled with the observed antimicrobial activity, underscores the promise of fungal-derived AgNPs in biomedical applications. Further research into their mechanisms of action and in vivo studies is warranted to explore their full therapeutic potential.

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