DETECTION AND MOLECULAR CHARACTERISATION OF PATHOGENIC ESCHERICHIA COLI AND SALMONELLA SPP IN RAW MEAT AND OFFALS IN MAGBORO, OGUN STATE

DETECTION AND MOLECULAR CHARACTERISATION OF PATHOGENIC ESCHERICHIA COLI AND SALMONELLA SPP IN RAW MEAT AND OFFALS IN MAGBORO, OGUN STATE

Abstract:

Escherichia coli and Salmonella are major foodborne pathogens causing severe disease in humans worldwide. The study was conducted to isolate, identify and characterize pathogenic E. coli and Salmonella from raw meat and offal collected from different meat vendors in Magboro, Ogun state. A total of three (3) samples were subjected to bacteriological isolation and identification, and the isolated bacteria were subjected to molecular typing. E. coli and Salmonella spp were isolated and identified by culturing on Eosin methylene blue agar, Sorbitol MacConkey agar, and Xylose Lysine Deoxycholate agar respectively. The total viable count was higher at 6 Log10 Cfu/g in all the samples while the highest count of pathogenic E. coli was 6.3 Log10 Cfu/g. The E. coli isolates were positive to 16S rRNA-based polymerase chain reaction (PCR). Salmonella species were found in 25 g of offal which is contrary to meat safety standards. The findings of this research project demonstrated the high level of microbial contamination and occurrence of pathogenic bacteria in raw meat and offal sold in Magboro, market, Ogun state.

Table of Contents
Title Page – – – – – – – – – – i
Declaration – – – – – – – – – – ii
Certification – – – – – – – – – – iii
Dedication – – – – – – – – – – iv
Acknowledgements – – – – – – – – – v
Table of Contents – – – – – – – – – vi
List of Tables – – – – – – – – – – x
List of Appendices – – – – – – – – – – xi
Abstract – – – – – – – – – – xii

 

CHAPTER ONE

INTRODUCTION

1.1 Background to the Study – – – – – – – – 1
1.2 Statement of the Problem- – – – – – – – 3
1.3 Research Questions- – – – – – – – – 5
1.4 Objectives of the Study- – – – – – – – – 6
1.5 Research Hypotheses- – – – – – – – – 6
1.6 Scope of the Study- – – – – – – – – 7
1.7 Significance of the Study- – – – – – – – 7

1.8 Limitations of the Study- – – – – – – – 8

CHAPTER TWO
LITERATURE REVIEW AND THEORETICAL FRAMEWORK
2.1 Introduction- – – – – – – – – – 9
2.2 General review
2.3 Review of related article
2.4 Review of Empirical Studies – 13
2.5 Theoretical Framework- – – – – – – – 18
2.6.1 Expectation Disconfirmation Theory- – – – – – – 19

 

CHAPTER THREE
METHODOLOGY

CHAPTER FOUR
DATA PRESENTATION AND ANALYSIS

CHAPTER FIVE
SUMMARY, CONCLUSION AND RECOMMENDATIONS

DETECTION AND MOLECULAR CHARACTERISATION OF PATHOGENIC ESCHERICHIA COLI AND SALMONELLA SPP IN RAW MEAT AND OFFALS IN MAGBORO, OGUN STATE

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