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ANALYSIS ON UROPATHOGENIC ESCHERICHIA COLI IN MEN’S RESTROOM FACILITIES AT A NIGERIAN TERTIARY INSTITUTION
Abstract:
Background: Urinary Tract Infections (UTIs) are among the most prevalent urinary tract maladies worldwide. Uropathogenic Escherichia coli (UPEC) strains are the primary culprits behind UTIs, equipped with various virulence factors for instigating urinary system infections. This research seeks to examine the prevalence of Uropathogenic E. coli among male students residing in hostels within a tertiary institution in Ogun state, Nigeria.
Objective: To identify Uropathogenic E. coli strains responsible for urinary tract infections and assess their impact on the health of male students.
Methodology: Using the swab-rinse technique, we randomly collected fifty samples from male hostel restrooms within a tertiary institution in Ogun state, Nigeria. These samples were cultured on MacConkey agar, and DNA was extracted from distinct isolates for multiplex polymerase chain reaction using primers targeting virulence genes, including papC, papEF, papGclassicI, Cdbt, and hlyA. Amplicons were visualized using a 1.8% agarose gel and UV transilluminator.
Results: Among the 50 samples examined, this study revealed a Uropathogenic E. coli prevalence of 44.6%. The most prevalent virulence gene was papC (42.2%), followed by hlyA (4.4%). PCR analysis identified 11 isolates carrying the PaPC gene (24.4%), 4 with PaPGclassic (8.8%), 3 with PaPE/F (6.7%), 2 with HlyA (4.4%), and 1 with Cdbt (2.2%). All strains tested negative for Usp and CNF. PaPC was identified as the most widespread virulence factor, occurring in 24.4% of the strains.
Conclusion: The findings of this study underscore the presence of Uropathogenic E. coli among the male student population, emphasizing its status as a prevalent cause of Urinary Tract Infections in Ogun state. This research provides valuable insights into the prevalence of UPEC within the male hostels of a tertiary institution. It underscores the importance of regular monitoring and screening for individuals displaying UTI symptoms to mitigate the spread of UPEC. Given the high prevalence of Uropathogenic E. coli, intervention measures may be warranted to control and prevent its dissemination.
